Bicolor’s Purple-Jelley Assay（透明質酸檢測試劑盒）采用Stains-all染料結合法定量檢測哺乳動物組織中的透明質酸。該方法詳細描述了在測定分離的透明質酸之前，通過兩步關鍵的電解質鹽析去除組織中蛋白和硫酸化糖胺聚糖的過程。

部分組織中透明質酸的分布情況：

Distribution of HA in Tissue

H1000透明質酸檢測試劑盒對樣本的要求：皮膚、軟骨、軟組織，包含不低于0.2ug的透明質酸。

Bicolor’s Purple-Jelley Assay（透明質酸檢測試劑盒）實驗操作步驟：

蛋白的去除：所有測試樣本都需要進行蛋白質消化。
糖胺聚糖的復蘇：通過GAG Precipitation Reagent 獲得釋糖胺聚糖。

透明質酸的分離測定：離心洗滌獲得透明質酸水合物后，繪制標準曲線，655nm處定量檢測透明質酸。

產品訂購：

Standard Assay Kit: Product Code H1000 (100 assays)

Economy Assay Kit: Product Code H2000 (400 assays)

參考文獻

1.Chemistry and Biology of Hyaluronan, Edited by H.G. Garg and C.A. Hales, (2004). Publisher: Elsevier Oxford, UK

2.Hyaluronan in Cancer Biology, Edited by R.Stern, (2009) Publisher: Academic Press, San Diego, U.S.A

3.In 1936 Edwin Jelley sent a ‘Letter to the Editor’ of Nature, (138 1009 -1010), regarding the unusual behaviour of some cyanine dyes.

4.When dissolved in 5 M NaCl these dyes produce a third absorbance peak at a longer wave-length (650nm) whereas in deionised water only a double peak occurs at ~540 nm and ~570 nm.

5.The 650 nm peak in concentration dye solution induces aggregation of the dye molecules and has been described as a ‘J-aggregate’ named after Jelley.

6.Individual dye molecules may form a stacking format to produce a supra-

molecular complex as seen in peak 3, (Image on front cover of the Manual)

7.Further studies in the 1960s notably by Kay et.al. (J. Physical Chem. 68 1896 – 1906) found many biological polymers including proteins, DNA, polar lipids and glycosaminoglycans could induce the third peak when using high dye concentrations.

8.The dye was renamed ‘Stains-all’ by Dhlberg, Dingman and Peacock in1969

( J. Mol. Biol. 41, 139).