Roche03353583910原裝 羅氏地高辛標記檢測試劑盒
參考價 | ¥ 5034 |
訂貨量 | ≥1 |
具體成交價以合同協議為準
- 公司名稱 廣州市齊云生物技術有限公司
- 品牌
- 型號 Roche03353583910原裝
- 產地 美國羅氏生物科學供應部
- 廠商性質 代理商
- 更新時間 2018/2/19 10:20:25
- 訪問次數 741
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Catalog # | Pack size | Qty | Price | Options |
---|---|---|---|---|
1 kit for 25 tailing reactions 100 pmol oligonucleotide per assay corresponding to 1 μg of a 30-mer oligonucleotide | 5034.00 |
一、使用原理:
Oligonucleotides, tailed with DIG-dUTP, are used for DNA or RNA hybridization in membrane blots or in situ hybridization.
二、產品優點:
- Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides
- Fast hybridization kinetics, due to the small size of oligonucleotides
- Single-stranded probes, no renaturation during hybridization
- Sequence can be designed according to the experiment
- Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells
三、產品描述:
Tailing of oligonucleotides at the 3'-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100).
四、試劑盒組成組分:
- Reaction Buffer, 5x, 100 µl
- CoCl2 Solution, 100 µl, 25 mM
- DIG-dUTP Solution, 25 µl, 1 mM
- dATP Solution, 25 µl, 10 mM
- Recombinant Terminal Transferase, 25 µl, [400 U/µl]
- Control Oligonucleotide, unlabeled, 25 µl, [20 pmol/µl]
- Oligonucleotide, DIG-dUTP/dATP tailed, 20 µl, [2.5 pmol/µl]
- Control DNA, 20 µl, [0.25 mg/ml]
- Glycogen Solution, 50 µl, [20 mg/ml]
- DNA Dilution Buffer, 3 x 1 ml, [50 µg/ml] fish sperm DNA
- poly(A) Solution, 1 ml, [10 mg/ml]
五:試劑盒檢測原理:
DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s).
六::,61288195 Mr SHen
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