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cellscript公司全系列產品
目前的產品包括用于體外轉錄的試劑盒、使用cap模擬物或cap酶的5' RNA capping和3' RNA聚腺苷酸化的試劑盒，以及用于制造用于細胞翻譯的cap、poly（a）-tail mRNA的all-in-one試劑盒。

cellscript公司全系列產品

 

 Cellscript™的使命是為臨床研究和治療提供*的產品和技術，用于制造和使用RNA在細胞中進行翻譯。

    目前的產品包括用于體外轉錄的試劑盒、使用cap模擬物或cap酶的5' RNA capping和3' RNA聚腺苷酸化的試劑盒，以及用于制造用于細胞翻譯的cap、poly(a)-tail mRNA的all-in-one試劑盒。

    Cellscript™近推出了隱身™的體外合成RNA的RNA轉錄套件包含修飾核苷,如假尿苷(Ψ)和/或5-methylcytidine (m5C)代替相應的U或C規范核苷(圖1)。這些工具都取名為“因為封頂,聚腺苷酸和核苷修飾的RNA產物(稱為“INCOGNITO mRNAs”)是經過偽裝的，因此當它們被轉染到表達多種RNA傳感器的哺乳動物細胞中時，不會像相應的未經修飾的mrna一樣，引發先天免疫反應。

產品清單：

 

cellscript公司全系列產品

 

mRNA Production

T7 mScript™ Standard mRNA Production System  (PDF)

Simply the best way to make in vitro mRNA. Combines the benefits of high-yield transcription with those of post-transcriptional capping and tailing all in one kit. See T7-FlashScribe Transcription Kit, ScriptCap m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus Poly(A) Polymerase Tailing Kit on this page.

C-MSC11610	10 reactions	$320
C-MSC100625	25 reactions	$702

 

In Vitro Transcription

T7-FlashScribe™ Transcription Kit  (PDF)

For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in 30 minutes.

C-ASF3507

50 reactions

$286

T7-Scribe™ Standard RNA IVT Kit  (PDF)

For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.

C-AS3107

50 reactions

$267

SP6-Scribe™ Standard RNA IVT Kit  (PDF)

For the production of in vitro transcribed RNA with SP6 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 90 µg RNA from 1 µg DNA in 2 hours.

C-AS3106

50 reactions

$267

Post-Transcriptional Capping

ScriptCap™ m7G Capping System  (PDF)

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching *. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce Cap 1 caps, that which is found on most eukaryotic mRNAs.

C-SCCE0625

25 reactions

$436

ScriptCap™ 2'-O-Methyltransferase Kit  (PDF)

For conversion of RNA containing Cap 0 caps to those containing Cap 1 caps, which are found on most eukaryotic mRNAs. Can be used simultaneously with ScriptCap m7G Capping System to produce virtually * Cap 1-capped RNA in a single reaction.

C-SCMT0625

25 reactions

$219

ScriptCap™ Cap 1 Capping System  (PDF)

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching *. This cannot be accomplished with co-transcriptional capping methods.

C-SCCS1710

10 reactions

$211

Co-Transcriptional Capping

MessageMAX™ T7 ARCA-Capped Message Transcription Kit  (PDF)

For co-transcriptional capping of IVT RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 80% correctly capped and 20% uncapped.

C-MMA60710

10 reactions

$323

AmpliCap-Max™ T7 High Yield Message Maker Kit  (PDF)

For co-transcriptional capping of IVT RNA with T7 RNA polymerase and standard cap analog. A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped.

C-ACM04037

25 reactions

$368

AmpliCap™ SP6 High Yield Message Maker Kit  (PDF)

For co-transcriptional capping of IVT RNA with SP6 RNA polymerase and standard cap analog. A reaction produces 35 µg RNA from 1 µg DNA in 2 hours. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped.

C-AC0706

25 reactions

$368

Post-Transcriptional Tailing

A-Plus™ Poly(A) Polymerase Tailing Kit  (PDF)

For post-transcriptional poly(A) tail addition to RNAs. Allows tailing of mRNAs in vitro, to lengths equivalent to or greater than those found in nature. This cannot be accomplished with co-transcriptional tailing methods.

C-PAP5104H

50 reactions

$219

Modified NTP In Vitro Transcription

INCOGNITO™ T7 Ψ-RNA Transcription Kit  (PDF)

For the production of pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours.

C-ICTY110510

10 reactions

$410

INCOGNITO™ SP6 Ψ-RNA Transcription Kit  (PDF)

For the production of pseudouridine-containing, in vitro transcribed RNA with SP6 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 60 µg RNA from 1 µg DNA in 2 hours.

C-ICSY110510

10 reactions

$410

INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit  (PDF)

For the production of 5-methylcytosine- pseudouridine-containing, in vitrotranscribed RNA with T7 RNA polymerase. 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours.

C-ICTMY110510

10 reactions

$691

INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit  (PDF)

For co-transcriptional capping of 5-methylcytosine- pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 40 µg RNA from 1 µg DNA in 3 hours. Reaction products are 80% correctly capped and 20% uncapped.

C-ICTAMY110510

10 reactions

$842

mRNA Decapping

Cap-Clip™ Acid Pyrophosphatase  (PDF)

For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer.

C-CC15011H

100 units

$550

RNase Inhibition

ScriptGuard™ RNase Inhibitor  (PDF)

Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction.

C-SRI6310K

10,000 units

$278