產品概述

Specificity ：GenScript’s THE™ PEG Antibody, mAb, Mouse has high affinity for the PEG backbone. The antibody binds to the variety of PEG such as PEG40K, PEG20K, PEG5K, PEG12, PEGylated drugs and PEG conjugates.

Host Species ：Mouse

Immunogen： PEG conjugated to KLH

Conjugate： Unconjugated

應用指導

Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.

Application：Recommended Usage

Sandwich ELISA： Capture: 5-10 µg/ml    Detection: 0.1-1.0 µg/ml

Competitive ELISA ：User-optimized

Western Blot ：0.1-1.0 µg/ml

Immunohistochemistry (IHC)： 10-15 µg/ml

產品屬性

Form： Lyophilized

Storage Buffer ：Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Reconstitution ：Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. For long-term storage, adding glycerol to a final concentration of 50% (v/v) in the reconstituted antibody solution is recommended.

Storage Instructions ：The lyophilized product remains stable for up to 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Purification： Affinity chromatography

Isotype： Mouse IgM

Clonality： Monoclonal

Clone ID： 5E10E9

Note： Some reagents such as Tween-20, Triton X-100 and NP-40 interfere with the PEG antibody interactions due to their structural similarity to PEG. Antibody solution should not contain such reagents in the assay of PEGylated molecules.

GenScript can customize this product per customer's request including product size, buffer components, etc.

技術背景

Target Background ：PEG (Polyethylene glycol) is a polyether compound with many applications from industrial manufacturing to medicine. PEGylation is a technology that covalently couples non-toxic, hydrophilic polyethylene glycol (PEG) to the drug. It is an FDA-approved method for the delivery of protein drugs. PEG modification can reduce the drug immunogenicity and antigenicity. PEGylated drug decelerates renal excretion, improves stability towards proteolysis and increases its half-life in blood. Accurate and sensitive quantification of PEG conjugates is important for PEG conjugated product development and pharmaceutical study. Polyethylene glycol (PEG) antibody is a useful tool for the detection of PEGylated molecules.

Synonyms ：Anti-Polyethylene glycol antibody, mAb, Mouse

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.