大鼠熱休克蛋白27(HSP-27)檢測

北京方程生物公司銷售Hu人環磷酰胺(CTX)試劑盒，英文名稱：Human Cyclin-D2 ELISA Kit

After incubation, transfer the reaction mixtures to the second antigen-coated microtitre plate. The ELISA assay using this second plate will now be performed exactly as for the first microtitre plate. The purpose of the second ELISA assay is to check that only a small fraction of the free antibody is captured on the first microtitre plate and, therefore, no readjustment of the equilibrium occurred during the first capture step. Wash extensively the first ELISA plate and perform the remaining steps of an ELISA procedure, aimed at the determination of the antibody binding to the coated antigen. Develop the ELISA with a suitable chromogenic, fluorogenic or chemiluminescent substrate, and measure the individual wells with an appropriate ELISA plate reader. The highest ELISA signal should be observed at low concentrations of antigen. No ELISA signal should be observed at high concentrations of antigen. The concentration of antigen at which the half-maximal ELISA signal is detected corresponds to the dissociation constant Kd. Alternatively, the Kd value can be obtained by fitting the ELISA signal of the individual wells to the equation: Kd = [A][B]/[AB] .;Competitive ELISA1. Coat Nunc immuno-module plates overnight, in usual manner. 2. Set up competition assay between antibody and competing substance :- Prepare a 1:2 serial dilution of the competitive substance from 480 to 0.4 ng/ml. Add 50ul of each of the above dilutions to 25ul of 3x strength titre of antibody (i.e. a solution 3 x the titre giving 50% inhibition on direct ELISA), in sealed tubes. 3. Incubate overnight at room temperature. 4. Set up competition between unknown concentration of competitive substance and antibody as above.

Mo小鼠CD3分子(CD3)

大鼠抗紅細胞抗體(RBC)

雞免疫球蛋白A(IgA)

Hu人FMS樣酪氨酸激酶3(Flt3)

Human Alpha-fetoprotein,AFP ELISA Kit

Hu人可溶性CD28(sCD28)

Human Legionella pneumophila antigen,LP Ag ELISA Kit

Pig豬心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)

Hu人膠原蛋白Ⅱ(HCBⅡ)

Hu人百日咳du素(PT)

Mouse Ceruloplasmin,CP/CER ELISA Kit

Mo小鼠肌紅蛋白(MYO/MB)

大鼠γ氨基丁酸(GABA)

Rat ferritin,FE ELISA Kit

Cover the plate with Parafilm and incubate for 2 hours at 37°C, followed by an overnight incubation at 4°C. Cover the plate tightly to avoid evaporation. Store at 4°C. The plate(s) will remain usable for about one month.

大鼠熱休克蛋白27(HSP-27)檢測咨詢