ACE （angiotensin converting enzyme） antibody

Description

Angiotensin-converting enzyme （ACE） is a zinc-containing dipeptidyl carboxypeptidase anchored in the plasma membrane with a carboxy-terminal hydrophobic domain （1, 2）. ACE has a critical role in cardiovascular function because it catalyses the conversion of angiotensin I to the physiologically active vasopressor octapeptide angiotensin II, which controls fluid-electrolyte balance and systemic blood pressure （3, 4）. Inhibitors of ACE are a first line of therapy for hypertension, heart failure, myocardial infarction and diabetic nephropathy （4）. ACE also plays a critical role in the circulating or endocrine renin-angiotensin system （RAS） as well as the local regulation that exists in tissues such as the myocardium and skeletal muscle （5）.

Recommended Applications

WB, IHC, FC

Applications and Recommended Dilution Factors

WB: 1:1000 - 10,000
IHC: 1:100 - 250
FC: 1:30

Species Reactivity *

Human, Mouse

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data

A. Western blot analysis on （A） fetal kidney, （B） fetal heart and （C） fetal lung lysates using anti-ACE （angiotensin converting enzyme） RabMAb, dilution 1:1000.	B.Immunohistochemical analysis of paraffin-embedded human kidney using anti-ACE （angiotensin converting enzyme） RabMAb .	C.Immunohistochemical analysis of paraffin-embedded spleen using anti-ACE （angiotensin converting enzyme） RabMAb Note positive staining of endothelial cells .

Specificity

A synthetic peptide corresponding to residues on the C-terminus of human ACE was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ACE antibody, ACE1 antibody, CD143 antibody, DCP antibody, DCP1 antibody, MGC26566 antibody, MVCD3 antibody, Angiotensin-converting enzyme, soluble form antibody, Dipeptidyl carboxypeptidase I antibody, Kininase II antibody

Description References

1. Ehlers MR, et al. Proc Natl Acad Sci U S A. 86（20）:7741-5,1989
2. Sugimura K, et al. Biochem Biophys Res Commun. 247（2）:466-72, 1998
3. Rieder MJ, et al. Nat Genet. 22（1）:59-62, 1999
4. Natesh R, et al. Nature. 421（6922）:551-4, 2003
5. Natesh R, et al. Biochemistry. 43（27）:8718-24, 2004