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[供應]CRL-1646 FO 小鼠骨髓瘤細胞

貨物所在地:上海上海市

更新時間:2025-05-09 21:00:07

有效期:2025年5月9日 -- 2025年11月9日

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CRL-1646 FO 小鼠骨髓瘤細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*培養條件
CRL-1646 FO 小鼠骨髓瘤細胞 的詳細介紹

CRL-1646 FO 小鼠骨髓瘤細胞

ATCC® Number:  CRL-1646™      Price:  $355.00 
Designations:  FO 
Depositors:   SF de St. Groth 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: suspension
Organism: Mus musculus (mouse) 
Morphology: lymphoblast

 
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Antigen Expression: H-2d
Comments: The line was line created by self fusing Sp2/0 myeloma cells using Sendai virus.
The cells are resistant to 8-azaguanine and do not produce immunoglobulin.
The line can be used a fusion partner for making B cell hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing:  Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 to 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation:  culture medium 95%; DMSO, 5%
Doubling Time:  8 to 10 hrs 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1092: de St.Groth SF, Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J. Immunol. Methods 35: 1-21, 1980. PubMed: 7009747
 Related Links  
NCBI Entrez Search 
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