CL-101 LLC-PK1 豬腎細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*培養條件
CL-101 LLC-PK1 豬腎細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*培養條件
CL-101 LLC-PK1 豬腎細胞 的詳細介紹
| ATCC® Number: | CL-101™ | Price: | |
| Designations: | LLC-PK1 | | Depositors: | Eli Lilly & Co. | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | | | | Organism: | Sus scrofa （pig） | | Morphology: | epithelial
| | Source: | Organ: kidney
Strain: Hampshire
Disease: normal | | Cellular Products: | plasminogen activator | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | | | Age: | 3 to 4 weeks | | Gender: | male | | Propagation: | ATCC complete growth medium: The base medium for this cell line is Medium 199 containing 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 3%.
Temperature: 37.0°C | | Subculturing: | Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. | | Preservation: | culture medium 95%; DMSO, 5% | | Related Products: | recommended serum:ATCC 30-2020
formerly distributed as:ATCC CRL-1392 | | References: | 3520: Hull RN, Huseby RM. Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975
22659: Perantoni A, Berman JJ. Properties of Wilms' tumor line （TuWi） and pig kidney line （LLC-PK1） typical of normal kidney tubular epithelium. In Vitro 15: 446-454, 1979. PubMed: 225262
28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 |
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