亚州一区二区三区中文字幕国产精品-日韩人妻一区二区三区蜜桃视频-亚洲中文字幕久久无码精品-午夜精品亚洲一区二区三区嫩草-日韩人妻一区二区三区蜜桃视频-国产一区二区精品高清在线观看-国产欧美日韩综合精品一区二区

文獻題目  Photoperiod affects Harderian gland morphology and secretion in female Cricetulus barabensis: Autophagy,apoptosis, and  mitochondria

作者  Zhe Wang, Jin-Hui Xu, Jun-Jie Mou，et al.

作者單位  曲阜師范大學

文獻中引用產品  倉鼠褪黑素（MT）ELISA試劑盒

關鍵詞  photoperiod, Harderian gland, autophagy, apoptosis, mitochondria

DOI   doi.org/10.3389/fphys.2020.00408

影響因子(IF)  3.22

出版期刊   《Frontiers in Physiology》

產品原文引用

Sample PreparationHamsters were sacrificed by CO2 asphyxiation at 22:00 on the last day, after all hamsters had been in darkness for at least 2 h. Bloodsamples were collected immediately from the heart after sacrifice,stored at 4?C for 30 min, and then centrifuged at 3000 rpm for 15 min at 4?C. Serum MT levels were estimated using an enzyme-linked immunosorbent assay (Shanghai Hengyuan Biological Technology Co., Ltd., H-40277, Shanghai, China). The HGs were removed, with lengths and weights then recorded. The HGs from five hamsters were fixed by immersion and processed for light and electron microscopy. The right HGs were immersed in 10% buffered formalin for paraffin section embedding, andthe left HGs were immersed in glutaraldehyde-paraformaldehyde for transmission electron microscopy (TEM). The remaining HGs from 15 hamsters in each group were frozen in liquid nitrogen and stored in a refrigerator at ?80℃ for subsequent Western blotting, real-time polymerase chain reaction (RTPCR), and immunofluorescence histochemical analyses. In each experiment, unilateral HGs from 10 hamsters were used for quantification. All procedures were carried out in accordance with approved guidelines.

完整版PDF文獻請咨詢我司業務員或者點擊下載！

更多公司福利請關注“恒遠生物”微xin公眾號！