亚州一区二区三区中文字幕国产精品-日韩人妻一区二区三区蜜桃视频-亚洲中文字幕久久无码精品-午夜精品亚洲一区二区三区嫩草-日韩人妻一区二区三区蜜桃视频-国产一区二区精品高清在线观看-国产欧美日韩综合精品一区二区

【文獻標題】 The distinct role of CD73 in the progression of pancreatic cancer
【作者】Liangjing Zhou1，Shengnan Jia1，Yan Chen，et.al
【作者單位】浙江大學（Zhejiang University）
【文獻中引用產品】
人腫瘤壞死因子α（TNF-α）ELISA試劑盒
【關鍵詞】Pancreatic cancer，Progression，CD73，TNFR2，miR-30a-5p
【DOI】doi.org/10.1007/s00109-018-01742-0
【影響因子(IF)】4.14
【出版期刊】 《Journal of Molecular Medicine》
【產品原文引用】 
Detection of TNF-α levels in supernatant via ELISA
Supernatant was investigated for levels of TNF-α protein using commercial TNF-α ELISAs (Hengyuan Biotechnology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions.Western blot Cells were harvested, and total protein lysates were resolved using 10% sodium dodecyl sulfate–polyacrylamide gels,electroblotted onto a polyvinylidene fluoride membrane,blocked by 5% skim milk, and probed with primary antibodies listed in Table S1. The membrane was then incubated with the secondary antibody and visualized by enhanced chemiluminescence using Kodak X-Omat LS film (Eastman Kodak,Rochester, USA).

完整版PDF文獻請咨詢在線客服或者電話聯xi我司業務員！
更多公司福利請關注“恒遠生物”VX公眾號！