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微型組合PH微電極
The microelectrode is ready to use. Carefully unwind the tape and remove the probe from the
protective glass tube. The white translucent sleeve, which covers the hole, should be moved
down

微型組合PH微電極

Calibration
The probe can be held by hand when measuring pH. It has been standardized in two (2) pHbuffers. Follow the procedure recommended by the manufacturer of your pH meter for
calibrating your pH meter with our microelectrode

微型組合PH微電極

Optimum Response Time: Optimum response time will be obtained after the probe has beenexercised in two (2) buffer solutions. Fill one beaker with a pH 4 buffer or equivalent and asecond beaker with a pH 7 buffer or equivalent. Hold the microelectrode and touch the pH 4buffer surface allowing 5 seconds forequilibration, and then touch the pH 7 buffer surface in thesame manner. Do this several times. It is not necessary to rinse the probe because the carryoveris negligible

微型組合PH微電極

The outer reference chamber of the microelectrode can be refilled when necessary with the 3 M
KCl solution, using the bulb pipette. The reference chamber should be filled slowly.
Be careful not to apply pressure against the inner glass capillary tube.
Cleaning
When using the microelectrode in solutions containing protein, it should be soaked in an enzyme
cleaning solution such as Terg-a-zyme (Alconox, Inc.) or a chromic/sulfuric acid glass cleaning
solution for a couple of minutes after each use to remove the protein from the glass and the
reference junction. This will prolong the useful life of the microelectrode.
Storage
Always clean the microelectrode before storing:
Long-term (over 2 weeks): Return the probe to its original container and prepare it in the same
condition in which you received it. Usually this means simply moistening the sponge located in
the bottom of the protective glass tube with pH 4 buffer.
Short-term: The probe can be left in an acid pH buffer solution (pH 4.01).