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DynaGen Automated Seeding Module*


 

The versatile DynaGen seeding module （patent pending） can be used with the DynaGen bioreactor chambers to seed constructs with up to 2 separate cell suspensions in a user defined pattern. The module design allows installation of the scaffold and cell seeding independent of the stimulation system.

For example, the fully automated seeding module can be integrated with the standard LumeGen bioreactor chambers （one configuration is pictured right） to seed tubular scaffolds. Dual sterilizable cannulas with controlled pumps deposit cells inside and outside of a graft with a user defined speed and pattern. New modified grips allow the graft to rotate independent from the chamber at a user defined speed. Once seeding has been completed the chamber can be plugged into the LumeGen bioreactor for stimulation.

Sample ID: 3mm - 6mm （for inner seeding applications）
Seeded Length: 40mm - 60mm （total graft length 55mm - 65mm）

The CellWorks software and control platform acommodates a wide range of user defined cell seeding protocols. The intuitive software runs under Windows XP. Step by step instructions prompt the user to input parameters, for example; sample length. desired spacing between cell suspension droplets, and rest periods. Other parameters such as seeding speed and density can be hardcoded in to the software to ensure precise repeatability of the seeding process. The CellWorks software displays seeding parameters and tracks the seeding progress. Linear displacement and rotation of the sample can be controlled individually, the dispersal rate of the syringes can also be controlled individually. These capabilities provide the user with an almost infinite number of seeding patterns, while still offering micron level positioning of the graft and tenths of degree rotation accuracy.

*Developed in Collaboration with the Institute for Biological Sciences of the National Research Council Canada

Products

Accessories

TGT also serves the broader tissue market with bioreactors and components for mechanically stimulated tissue growth.

Whether the research is on undifferentiated cells, simple 2-D flow systems, or complex 3-D organ-scaffolds, TGT can provide the systems, reactors, and components necessary for your research. In addition to bioreactor chambers, stimulators and system controllers, TGT can also supply your lab with the following accessories:

• Media reservoirs
• Non-contact laser micrometers
• Transducers
• Cell seeding methods
• Custom chambers & stimulator designs

Does your research require a hydrogel? Glycosan Biosystems offers Extracel^TM Hydrogels, designed for 3-D cell culture and tissue growth. Extracel^TM is a semi-synthetic hydrogel that is biocompatible, biodegradable and customizable. Click here to learn more.