描述

Dynabeads® M-270 Streptavidin the gold standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is used in a vast number of applications. Benefits and features:

• Direct and fast isolation of any biotinylated molecule
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Very low nonspecific binding of nucleotides and nucleic acids
• Very low aggregation in high-salt hybridization buffers
• Well suited for nucleic acid applications with extreme demands
• Low nonspecific binding of small and negatively charged proteins
• Production follows a validated process in compliance with cGMP for medical devices
• Well suited for automated protocols

About Dynabeads® M-270 Streptavidin
These uniform and superparamagnetic beads are 2.8 µm in diameter, with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface. This leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. They are hydrophilic, negatively charged and show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results. Applications
Over the past 15 years, streptavidin-coupled Dynabeads® have been used and cited for a very wide variety of applications. Examples include direct/indirect isolation and downstream handling of nucleic acids, proteins/peptides, and other target molecules. Ideal for sequence-specific DNA/RNA capture in nucleic acid–based diagnostics, specifically with samples with a high chaotropic salt concentration, immunoassays involving small biotinylated antigens, and applications that are not compatible with BSA (these beads are not blocked with BSA). Easily adaptated to automated processes. Dynabeads® are used on more than 25,000 routine IVD instruments worldwide. The product holds high standards with respect to reproducibility (both within and between batches), and automation ability, and drives reliability for your results.

Binding capacity
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® M-270 Streptavidin typically binds:

• >950 pmoles free biotin
• ~200 pmol biotinylated peptides
• ~10 µg biotinylated IgG
• ~10 µg ds-DNA
• ~200 pmol ss-oligonucleotides

圖表

Highly sensitive HCV ssDNA capture.

Monodisperse superparamagnetic 2.8 µm Dynabeads®.

規格

內容及儲存

This Product Contains: Dynabeads® (2.8 µm) with covalently coupled recombinant
streptavidin. Supplied in PBS, pH 7.4 with 0.02% NaN3.

Store at: 2-8°C.