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Pfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5′→3′ direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90,000 daltons as estimated from the predicted amino acid sequence and exhibits 3′→5′ exonuclease （proofreading） activity. Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity.