 | 培安有限公司 |
會(huì)員.png)
010-65528800
高 （市場(chǎng)部）
- 電話：
- 010-65528800
- 手機(jī)：
- 售后電話：
- 010-64278205
- 傳真：
- 010-65519722
- 聯(lián)系我時(shí)，
- 告知來自化工儀器網(wǎng)
- 個(gè)性化：
- www.pynnco.net
- 商鋪網(wǎng)址：
- http://www.wanmeizu.com/st26894/
- 公司網(wǎng)站：
- http://www.pynnco.com
壓力循環(huán)技術(shù)（PCT）為弗蘭克氏菌蛋白的研究提供方便
閱讀：1670發(fā)布時(shí)間：2009-02-27
| 提 供 商： | 培安有限公司 | 資料大小： | 125.604B |
|---|---|---|---|
| 資料圖片： | 下載次數(shù)： | 355次 | |
| 資料類型： |
PDF 文件  |
瀏覽次數(shù)： | 1670次 |
Frankia are gram-positive, filamentous, nitrogen-fixing actinobacteria that are symbiotic with over 200
different species of plants. Frankia produce three cell types: vegetative hyphae, spores located in
sporangia, and the unique lipid-enveloped cellular structures, termed vesicles. Vesicles are formed
inside plant cell nodules, or in culture under nitrogen limiting conditions, and act as specialized structures
for the nitrogen fixation process. The mature vesicle is surrounded by an envelope that extends down the
stalk of the vesicle past the basal septum, which separates the vesicle from the hyphae. Techniques have
been developed for the isolation and urification of intact vesicles from Frankia grown in culture [1,2,3]. Initial
investigations on the properties of purified vesicles have focused on nitrogen metabolism [3,4]. Protein
extraction from vesicles has historically been difficult and a significant bottleneck to proteomic studies
because vesicles are resilient structures that are difficult to disrupt. Reliable and comprehensive proteomic
maps of Frankia hyphae and vesicles can only be assured when proteins are isolated reproducibly and in
a manner in which they are accuray represented in the downstream analysis. For example, 2DGE can be
an accurate representation of a proteome only if the entire protein constituency of cells is recovered during
the sample preparation process. While French press treatment effectively lyses hyphae, it fails to disrupt
Frankia vesicles. Pressure Cycling Technology （PCT） used in combination with a specialized lysis reagent
effectively disrupted purified vesicles enabling further investigation of the proteome of the vesicles.
以上信息由企業(yè)自行提供，信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé)，化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。 溫馨提示：為規(guī)避購買風(fēng)險(xiǎn)，建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。
