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Acclaim Trinity P2 LC Column

The Thermo Scientific? Acclaim? Trinity? P2 column is a high-performance, silica-based column specifically designed for separation of pharmaceutical mono- and multi-valent counterions by high-performance liquid chromatography (HPLC). This column was engineered for analytical chemists who need simple, robust, fast, generic methods. The Acclaim Trinity P2 columns provide an effective solution for counterion analysis using one column and one method on a standard HPLC instrument.

? Desired selectivity for pharmaceutical mono- and multi-valent counterion screening
? Retention of ionic and ionizable analytes without using ion-pairing reagents
? Compatibility with charged aerosol and mass spectrometry detection methods
? Easy to use
? Rugged packing

The Acclaim Trinity P2 column is based on nanopolymer silica hybrid (NSH) technology.  It consists of high-purity, porous, 3 μm particles coated with charged nanopolymer particles: the innerpore area of the silica particles is modified with a covalently bonded hydrophilic layer that provides cation exchange retention, while the outer surface is modified with anion-exchange nanopolymer beads. This chemistry design ensures spatial separation of the anion exchange and cation exchange regions. In addition, its hydrophilic surface makes it useful as a hydrophilic interaction liquid chromatography (HILIC) column. Thus, the Acclaim Trinity P2 column provides cation-exchange, anion-exchange, and HILIC retentions on the same stationary phase.

NSH Chromatographic Features

• Multiple retention mechanisms including HILIC, anion-exchange, and cation-exchange
• Adjustable selectivity by phase ionic strength, electrolyte type, pH, and organic solvent
• Ideal selectivity for simultaneous separation of basic, neutral, and acidic analytes
• Separation of hydrophilic ionic and ionizable analytes without ion-pairing reagents

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