Biocolor 可溶性膠原檢測試劑盒
Biocolor Sircol Soluble Collagen Assay 

    采用與膠原特異性結合的Sircol染料，主要用于定量檢測哺乳動物組織中抽提的酸溶性膠原和體外培養哺乳動物細胞釋放到培養基中的膠原。

    溶于酸、胃蛋白等的可溶性膠原均可檢測，具體可檢測的膠原類型為I-V型，VI-XIV型膠原也可檢測，但由于難以制備標準曲線，還無法定量。Sircol染料不能區分不同類型的膠原，但可與膠原的[Gly-X-Y]n螺旋結構特異性地結合。隨著膠原變性為凝膠，該染料與膠原的結合力減弱。非脊椎動物與膠原由于變性溫度較低、羥脯氨酸殘基較少等原因，結合染料的能力較弱，需要制備種屬特異性的純化標準品，以繪制標準曲線。
  
實驗步驟

1. 繪制標準曲線
2. 待測樣本的制備：取50ul樣本容易調整體積到100ul進行反應。根據反應的結果對樣本濃度進行調整，使其在標準曲線范圍內；
3. 膠原—染料復合物的獲得：加入1ml Sircol染料，混勻離心后，既得膠原—染料復合物，棄上清，去掉未結合的染料
4. 加入1ml 染料釋放劑，將膠原—染料復合物中的染料釋放出來
5. 吸光度測定：用分光光度計540nm出定量測定。或使用colorimeter測定

產品訂購 
    Product Code: S1000    120 assays 
    Product Code: S5000    475 assays

參考文獻 
1
Anilkumar, T. V., Vineetha, V. P., Revi, D., Muhamed, J. and Rajan, A. (2014) Biomaterial properties of cholecystderived scaffold recovered by a non-detergent/enzymatic method. Journal of Biomedical Materials Research Part B:
Applied Biomaterials.
2
Arizmendi, N., Puttagunta, L., Chung, K. L., Davidson, C., Rey-Parra, J., Chao, D. V., Thebaud, B., Lacy, P. and
Vliagoftis, H. (2014) Rac2 is involved in bleomycin-induced lung inflammation leading to pulmonary fibrosis. Respiratory
research 15, 71.
3
Aschner, Y., Khalifah, A. P., Briones, N., Yamashita, C., Dolgonos, L., Young, S. K., Campbell, M. N., Riches, D. W.,
Redente, E. F., Janssen, W. J., et al. (2014) Protein Tyrosine Phosphatase α Mediates Profibrotic Signaling in Lung
Fibroblasts through TGF-β Responsiveness. The American journal of pathology 184, 1489–1502.
4
Banon-Maneus, E., Rovira, J., Ramirez-Bajo, M. J., Moya-Rull, D., Hierro-Garcia, N., Takenaka, S., Diekmann, F.,
Eickelberg, O., Königshoff, M. and Campistol, J. M. (2014) Wnt Pathway Activation in Long Term Remnant Rat Model.
BioMed Research International 2014.
5
Bellaye, P.-S., Burgy, O., Colas, J., Fabre, A., Marchal-Somme, J., Crestani, B., Kolb, M., Camus, P., Garrido, C. and
Bonniaud, P. (2014) Anti-fibrotic Role of αB-crystallin Inhibition in Pleural and Subpleural Fibrosis. American journal of
respiratory cell and molecular biology.
6
Bellaye, P.-S., Wettstein, G., Burgy, O., Besnard, V., Joannes, A., Colas, J., Causse, S., Marchal-Somme, J., Fabre, A.,
Crestani, B., et al. (2014) The small heat-shock protein αB-crystallin is essential for the nuclear localization of Smad4:
impact on pulmonary fibrosis. The Journal of pathology 232, 458–472.
7
Beyer, C., Zenzmaier, C., Palumbo-Zerr, K., Mancuso, R., Distler, A., Dees, C., Zerr, P., Huang, J., Maier, C.,
Pachowsky, M. L., et al. (2014) Stimulation of the soluble guanylate cyclase (sGC) inhibits fibrosis by blocking noncanonical TGFβ signalling. Annals of the rheumatic diseases annrheumdis–2013.
8
Biancheri, P., Di Sabatino, A., Ammoscato, F., Facciotti, F., Caprioli, F., Curciarello, R., Hoque, S. S., Ghanbari, A.,
Joe-Njoku, I., Giuffrida, P., et al. (2014) Absence of a role for interleukin-13 in inflammatory bowel disease. European
journal of immunology 44, 370–385.
9
Birukawa, N. K., Murase, K., Sato, Y., Kosaka, A., Yoneda, A., Nishita, H., Fujita, R., Nishimura, M., Ninomiya, T.,
Kajiwara, K., et al. (2014) Activated hepatic slate cells are dependent on self collagen, cleaved by membrane type
1-matrix metalloproteinase for their growth. Journal of Biological Chemistry jbc–M113.
10
Bode, C., Kinjo, T., Alvord, W. G. and Klinman, D. M. (2014) Suppressive oligodeoxynucleotides reduce lung cancer
susceptibility in mice with silicosis. Carcinogenesis bgu005.
11
Chen, M.-H., Hu, C.-K., Chen, P.-R., Chen, Y.-S., Sun, J.-S. and Chen, M.-H. (2014) Dose-dependent regulation of cell
proliferation and collagen degradation by estradiol on ligamentum flavum. BMC musculoskeletal disorders 15, 238.12
Chen, T., Nie, H., Gao, X., Yang, J., Pu, J., Chen, Z., Cui, X., Wang, Y., Wang, H. and Jia, G. (2014) Epithelial–
mesenchymal transition involved in pulmonary fibrosis induced by multi-walled carbon nanotubes via TGF-beta/Smad
signaling pathway. Toxicology letters 226, 150–162.
13
Chen, Y.-S., Lee, S.-M., Lin, Y.-J., Chiang, S.-H. and Lin, C.-C. (2014) Effects of Danshensu and Salvianolic Acid B from
Salvia miltiorrhiza Bunge (Lamiaceae) on Cell Proliferation and Collagen and Melanin Production. Molecules 19, 2029–
2041.
14
Chen, Y.-P., Chen, W.-C., Wang, K.-C. and Chen, C.-H. (2014) Effectiveness of synovial fluid mesenchymal stem cells
embedded in alginate beads for treatment of steroid-induced avascular necrosis of the femoral head. Journal of
Orthopaedic Science 1–10.
15
Choi, E. M. (2014) Sunflower seed extract enhances the differentiation of osteoblastic MC3T3-E1 cells. Food and
Agricultural Immunology 25, 9–19.
16
Chung, C.-C., Hsu, R.-C., Kao, Y.-H., Liou, J.-P., Lu, Y.-Y. and Chen, Y.-J. (2014) Androgen Attenuates Cardiac
Fibroblasts Activations through Modulations of Transforming growth factor-β and Angiotensin II Signaling. International
Journal of Cardiology.
17
Conte, E., Iemmolo, M., Fagone, E., Gili, E., Fruciano, M., Genovese, T., Esposito, E., Cuzzocrea, S. and Vancheri, C.
(2014) Thymosin β4 reduces IL-17-producing cells and IL-17 expression, and protects lungs from damage in bleomycintreated mice. Immunobiology 219, 425–431.
18
Dakin, S. G., Smith, R. K. W., Heineg\a ard, D., Önnerfjord, P., Khabut, A. and Dudhia, J. (2014) Proteomic Analysis of
Tendon Extracellular Matrix Reveals Disease Stage-specific Fragmentation and Differential Cleavage of COMP (Cartilage
Oligomeric Matrix Protein). Journal of Biological Chemistry 289, 4919–4927.
19
Daryadel, A., Bogdanova, A., Gassmann, M., Mueller, X., Zünd, G., Seifert, B., Lehalle, C., Frossard, N. and Tavakoli,
R. (2014) Multikinase inhibitor sorafenib prevents pressure overload-induced left ventricular hypertrophy in rats by
blocking the c-Raf/ERK1/2 signaling pathway. Journal of Cardiothoracic Surgery 9, 81.
20
Den Hartog, G. J., Qi, S., van Tilburg, J. H., Koek, G. H. and Bast, A. (2014) Superoxide anion radicals activate hepatic
slate cells after entry through chloride channels: A new target in liver fibrosis. European journal of pharmacology