?TRIZOL試劑是直接從細胞或組織中提取總RNA的試劑。它在破碎和溶解細胞時能保持RNA的完整性。加入后離心，樣品分成水樣層和有機層。RNA存在于水樣層中。收集上面的的TRLZOLReagent （Ambion®） 總RNA提取試劑，invitrogen貨號15596-018
| 品牌： | Invitrogen |
| 貨號： | 15596-018 |
| 數(shù)量： | 大量 |
| 供應商： | invitrogen |
| 規(guī)格： | 100 UG/100ml/200ml |
TRLZOLReagent (Ambion®) 總RNA提取試劑，invitrogen貨號15596-018
Description
TRIzol® Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.
Key Features of TRIzol® Reagent:
• Isolate RNA, DNA, and protein from the same sample
• Superior lysis capability, even with difficult sample types
• Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria
Reliably Purify RNA from Multiple Sample Volumes and Sources
TRIzol® Reagent performs well with small quantities of tissue (50-100 mg) and cells (5 × 106), as well as with large quantities of tissue (≥1 g) and cells (>107), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin.
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TRLZOLReagent (Ambion®) 總RNA提取試劑，invitrogen貨號15596-018
| 貨號 | 名稱 | 規(guī)格 | | 13778-075 | Lipofectamine RNAiMAX | 0.75ml | | 13778-150 | Lipofectamine RNAiMAX | 1.5ml | | 10296-010 | Trizol ® LS Reagent | 100ml | |
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| 10296-028 | Trizol ® LS Reagent | 200ml | |
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| 18374-058 | 5' RACE System for Rapid Amplification of cDNA Ends, Version 2.0 | 10 reactions | | 18373-019 | 3' RACE System for Rapid Amplification of cDNA Ends | 20 reactions | | 18064-014 | SuperScript® II Reverse Transcriptase | 10,000U | | 18080-044 | SuperScript® III Reverse Transcriptase | 10,000U | | 18418-012 | Oligo(dT)12-18 Primer | 25ug | | 18418-020 | Oligo(dT)20 Primer | 50ul(50uM) | | 10777-019 | RNaseOUTTM Ribonuclease Inhibitor | 5,000U | | 18080-051 | SuperScript® III First-Strand | 50 rxn | | 11668-027 | Lipofectamine TM 2000 Reagent | 0.75ml | | 11668-019 | 1.5ml | | 15596-026 | TRlzol ®Reagent | 100ml | | 15596-018 | TRlzol ®Reagent | 200ml | | 11789020 | BP CLONASE II ENZYME MIX | 20 REACTIONS | | 11791020 | LR CLONASE II ENZYME MIX | 20 REACTIONS | | 28025-013 | M-MLV Reverse Transcriptase | 40,000U | | 17504-044 | B-27（黃顏色） | 10ml | | 12587-010 | B-27（黃顏色）不含維生素A | 10ml | | 17502-048 | N-2 (粉紅顏色) | 5ml |
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TRLZOLReagent (Ambion®) 總RNA提取試劑，invitrogen貨號15596-018
TRLZOLReagent (Ambion®) 總RNA提取試劑，invitrogen貨號15596-018
配制方法
在2000ml的燒杯中加入以下物質(zhì)，混合均勻：
500ml重蒸苯酚；
250g異硫氰酸胍；
293mL蒸餾水；
⒘6mL0.75M檸檬酸鈉溶液（pH≥7）
26.4mL10%sarcosy（十二烷基肌氨酸鈉）溶液
50mL2MNaAc溶液（pH≥4）
10
