9
當前位置：> 供求商機> A549-Dual KO-MAVS Cells
[供應(yīng)]A549-Dual KO-MAVS Cells 返回列表頁
貨物所在地：北京北京市
更新時間：2025-03-31 09:46:21
有效期：2025年3月31日 -- 2026年4月1日
已獲點擊：47
聯(lián)系我時，請告知來自 化工儀器網(wǎng)
您好, 歡迎來到化工儀器網(wǎng)！ 登錄| 免費注冊| 產(chǎn)品展廳| 收藏商鋪|
您好, 歡迎來到化工儀器網(wǎng)！ 登錄| 免費注冊| 產(chǎn)品展廳| 收藏商鋪|
貨物所在地：北京北京市
更新時間：2025-03-31 09:46:21
有效期：2025年3月31日 -- 2026年4月1日
已獲點擊：47
聯(lián)系我時，請告知來自 化工儀器網(wǎng)
A549-Dual™ KO-MAVS cells were generated from A549-Dual™ cells through the stable knockout of the MAVS gene. They are adherent epithelial cells derived from the human A549 lung carcinoma cell line by stable integration of two inducible reporter constructs.
The A549 cell line, a cellular model for asthma and respiratory infections, expresses many pattern recognition receptors (PRRs), including RIG-I [1,2], and the Toll-like receptors (TLRs) TLR2 [3], TLR3, and TLR5 but not TLR4 [3].
A549-Dual™ KO-MAVS and A549-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB binding sites.
They also express the secreted Lucia luciferase reporter gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements.
As a result, they allow to simultaneously study the NF-κB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.
請輸入賬號
請輸入密碼
請輸驗證碼
以上信息由企業(yè)自行提供，信息內(nèi)容的真實性、準確性和合法性由相關(guān)企業(yè)負責，化工儀器網(wǎng)對此不承擔任何保證責任。
溫馨提示：為規(guī)避購買風險，建議您在購買產(chǎn)品前務(wù)必確認供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。