9當前位置：> 供求商機> bb-ifng——Murine B16 melanoma IFN-γ reporter cells
當前位置：> 供求商機> bb-ifng——Murine B16 melanoma IFN-γ reporter cells
當前位置：> 供求商機> bb-ifng——Murine B16 melanoma IFN-γ reporter cells
B16-Blue™ IFN-γ cells derive from the murine B16 melanoma cell line of C57BL/6 origin after stable transfection with a SEAP (secreted embryonic alkaline phosphatase) reporter gene under the control of the IFN-inducible ISG54 promoter enhanced by a multimeric interferon-sensitive response element (ISRE).
B16-Blue™ IFN-γ cells do not respond to IFN-α/β, due to the inactivation of the type I IFN receptor. B16-Blue™ IFN-γ cells respond specifically to mIFN-γ and do not respond to human IFN-γ.
Stimulation of B16-Blue™ IFN-γ cells with mIFN-γ triggers the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™ Solution, a medium that turns purple/blue in the presence of SEAP and by reading the OD at 655 nm.
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