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蘇州螞蟻淘生物科技有限公司>技術文章>invitrogen/A11029/A11034二抗*

技術文章

invitrogen/A11029/A11034二抗*

閱讀:3547          發布時間:2017-12-29
ThermoFisherA11029Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate
ThermoFisherA11034Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate

invitrogen/A11029/A11034二抗*

訂貨找螞蟻淘就對了,4000-520-626

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-11034) in IF

Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/ml Rabbit primary antibody for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate (Product # A-11034) was used at a concentration of 4 µg/ml in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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螞蟻淘(ebiomall)是一家生命科學領域的垂直電商平臺,公司專注生物醫學科研用品的  導購和品牌推廣,為海外廠方與國內代理經銷商搭建跨境貿易系統和交流平臺。
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螞蟻淘常見平行進口產品有:
Thermofisher旗下Gibco培養系列、ABI細胞計數儀及PCR儀、Invitrogen分子生物學試劑及Qubit3.0、Ambion的RNA相關試劑、Molecular probes試劑、Pierce蛋白相關試劑盒、Nanodrop One/OneC;Parafilm封口膜;Viskase透析袋;Sigma-Aldrich試劑;AccuStandard 標準品;Millipore抗體;Glen Research 核酸合成試劑;Bio-Rad電泳儀及PCR儀;Nanocs的PEG聚合物;  Chromotek羊駝抗體;   Polysciences轉染試劑;  Echelon-inc 脂類研究試劑盒;   R&D Systems抗體及Elisa試劑盒; Jackson二抗及封閉血清; Biotium 熒光染料;NEB內切酶及NGS相關產品;  Athens Research & Technology人類蛋白及抗血清;  Illumina二代測試試劑盒; Tocris 小分子試劑; TwistDx恒溫擴增試劑盒(RPA); Enzymatics二代測序酶;Novus抗體及Elisa試劑盒;AG scientific潮霉素B、G-418;Plantmedia植物培養系列及抗體;Agilent 2100生物分析儀;Biolog耗材;Biomatik的Elisa試劑盒;Covaris基因打斷儀;CST抗體;Dharmacon的siRNA產品。

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