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Jackson|二抗209-545-097*
通過免疫親和層析從抗血清分離全I(xiàn)gG抗體作為完整分子。 它們具有Fc部分和兩個(gè)通過二硫鍵連接在一起的抗原結(jié)合Fab部分，因此它們是二價(jià)的。
通過免疫親和層析從抗血清分離全I(xiàn)gG抗體作為完整分子。 它們具有Fc部分和兩個(gè)通過二硫鍵連接在一起的抗原結(jié)合Fab部分，因此它們是二價(jià)的。
詳細(xì)介紹
Jackson|二抗209-545-097*
通過免疫親和層析從抗血清分離全I(xiàn)gG抗體作為完整分子。 它們具有Fc部分和兩個(gè)通過二硫鍵連接在一起的抗原結(jié)合Fab部分，因此它們是二價(jià)的。 據(jù)報(bào)道平均分子量約為160kDa。 整個(gè)IgG形式的抗體適用于大多數(shù)免疫檢測程序，并且是成本效益的。
基于免疫電泳和/或ELISA，抗體與人IgG的F（ab'）2 / Fab部分反應(yīng)。 它還與其他人免疫球蛋白的輕鏈反應(yīng)。 未檢測到針對人IgG的Fc部分或針對非免疫球蛋白血清蛋白的抗體。 該抗體顯示出對小鼠血清蛋白的固有的小交叉反應(yīng)，并且已通過ELISA和/或固相吸附測試以確保與牛，馬和小鼠血清蛋白的小交叉反應(yīng)。 抗體可以與來自其他物種的免疫球蛋白交叉反應(yīng)。
Jackson|二抗209-545-097*
Physical State: Freeze-dried solid
Storage:
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
1:100 - 1:800 for most applications
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.