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Source

Mouse

Applications

WB,IF,IHC

Reactivity

Human,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Pig,sheep,Insect,Yeast

Dilution

WB: 1:5000-20000, IHC: 1:200-300, IF 1:200

Storage

-20°C/1 year

Specificity

The antibody detects endogenous GAPDH protein.

Source/Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen

Synthetic Peptide of GAPDH

Uniprot No

P04406

Form

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Monoclonal

Background

glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri

Concentration

1 mg/ml