亚州一区二区三区中文字幕国产精品-日韩人妻一区二区三区蜜桃视频-亚洲中文字幕久久无码精品-午夜精品亚洲一区二区三区嫩草-日韩人妻一区二区三区蜜桃视频-国产一区二区精品高清在线观看-国产欧美日韩综合精品一区二区

武漢原生原代生物醫藥科技有限公司

PriCells: Primary dermal fibroblasts isolated from human

時間:2021-10-29 閱讀:233
分享:

PriCells: Primary dermal fibroblasts isolated from human                  


Fibroblasts were established from donors of human normal skin, scar, and keloid using the explant method.

The specimens were washed with sterile phosphate-buffered saline (PBS), and the subcutaneous tissues were removed carefully.

The skins were then cut into small pieces (1-2 mm3).

After being digested with PriCells Isolation Kit III (or using 0.25% trypsin containing 0.05% ethylenediaminetetraacetic acid) at 4°C overnight, the epidermal layers were removed, and the remaining dermal parts were further digested with PriCells Isolation Kit III (or using 0.25% trypsin containing 0.05% ethylenediaminetetraacetic acid) at 37°C for another 4 hours.

The digested cells were then passed through a 70-µm cell strainer (BD Biosciences), centrifuged, and resuspended in PriCells primary cell culture system.

Cells were seeded on tissue culture plates at 1x103 cells/cm2, and maintained at 37°C with 5% CO2.

After 24 hours, the plates were washed with PBS to remove residual non-adherent cells.

The resulting adherent cells were grown to confluency within 7 days.

Cells were subcultured every 4 days using 0.25% trypsin/EDTA.


會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復您~
撥打電話 產品分類
在線留言
主站蜘蛛池模板: 石棉县| 白山市| 仲巴县| 河北区| 武乡县| 家居| 盐池县| 榆林市| 宜州市| 深圳市| 宜兴市| 凤翔县| 天长市| 谢通门县| 鹤峰县| 南投县| 大余县| 华蓥市| 米林县| 江城| 信宜市| 当阳市| 延津县| 花垣县| 嘉定区| 克拉玛依市| 顺昌县| 星座| 崇义县| 明星| 高陵县| 蒙自县| 新竹市| 高阳县| 汪清县| SHOW| 东至县| 保亭| 齐齐哈尔市| 河池市| 阳新县|