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美國 Bio-rad轉基因儀基因槍系統(tǒng)Helios

顆粒轟擊用于體外和體內(nèi)基因轉移的優(yōu)點

* 簡單、快速、功能靈活的基因傳送，各種細胞類型通用
* 有助于瞬時表達和穩(wěn)定表達
* 只需少量的DNA 和細胞
* 無需載體DNA
* 能進行多質(zhì)粒共同導入
* 輸送大片段DNA
* 胞內(nèi)目標基因可導入多個細胞
* 用于體內(nèi)和體外轉化
* 沒有無關的基因或蛋白被轉導

The Helio gene gun is a convenient portable device for rapid and direct in-situ gene transfer of cells.The system USES an adjustable pulse of helium gas to drive gold particles of DNA, RNA or other biomaterials prepacked on the inside of a small plastic tube directly into a cell.The in-tube "bullet" can be easily prepared using the sample control station.

The advantages of particle bombardment for gene transfer in vitro and in vivo

* simple, fast, functional gene transfer across all cell types

* contributes to transient and stable expression

* only small amounts of DNA and cells are needed


* no carrier DNA is required

* multiple plasmids can be imported together

* transport large fragments of DNA

* target genes can be introduced into multiple cells

* for in vivo and in vitro transformation