BGC-823-LUC人低分化前胃癌細胞-熒光素酶標記 含STR

細胞英文(簡稱）：BGC-823、BGC823

細胞名稱：BGC-823、BGC823人胃癌細胞【已通過STR鑒定】

背景資料

該細胞源自一位62歲的胃癌（未分化腺癌）患者，表達CEA。 

細胞來源：ATCC

代次：P3

規格：T25

細胞數：1x10^6 cells

組織來源：胃癌

BGC-823、BGC823人胃癌細胞【已通過STR鑒定】形態：貼壁；上皮細胞樣

細胞活力：95％（Viability by Trypan Blue Exclusion）

細胞檢測：細胞不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌

培養條件：RPMI-1640 +10% FBS；37℃，5% CO2

傳代方法：*建議1:2-1:3 兩天換液一次

凍存條件：90%FBS+10%DMSO

動物細胞培養,大致的步驟是這樣:

1.從動物胚胎或者一些剛出生的動物的器官或者組織上取得細胞,配制成細胞懸浮液.把細胞液放到培養瓶中,在培養箱里培養--------原代培養.

但是,不要以為這樣就能一直無限培養下去.

因為BGC-823、BGC823人胃癌細胞【已通過STR鑒定】在那個瓶壁上生長的時候,如果大量增殖,細胞之間會彼此相碰,細胞細胞就會停止分裂增殖,出現一種接觸抑制.

2.我們為了它們能繼續增殖,就用胰蛋白酶再把它們分開,然后再配制成細胞懸浮液,分開裝到好幾個瓶子里繼續培養--------傳代培養.

細胞株&細胞系

1.它們都是傳代培養里面的概念.都是10代以后的

2.細胞株是傳代培養里10~50（不同物種不同組織細胞有差別吧）代,細胞系是50代以后的;

3.對于細胞繁殖而言,傳到10代就很不容易了,所以細胞株是極少數的細胞

4.50代以后,細胞繁殖會出現另一個危機,基本上沒有什么細胞能再傳下去了.但是,有細胞發生了基因突變,像一個癌細胞一樣無限地分裂下去,這就是細胞系.

BGC-823-LUC人低分化前胃癌細胞-熒光素酶標記 含STR-ATCC保藏中心
【Organism物種來源】Animal 
【Tissue組織來源】 
【Cell Type細胞類型】 
【Product Format產品狀態】 frozen/live culture
【Morphology細胞形態】 
【Culture Properties細胞特性】 
【Biosafety Level生物安全級別】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

【Disease細胞源疾病】
【Age年齡】 
【Gender性別】 Male/Female
【Storage Conditions保存條件】 liquid nitrogen vapor phase
【Karyotype染色體組型】 
【Images細胞圖片】 Cell Micrograph
【Derivation衍生細胞】
【Clinical Data臨床數據】 
【Comments其他描述】 
【Complete Growth Medium*培養基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 
【Subculturing傳代方法】 
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation凍存條件】 
【Freeze Medium凍存培養基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存溫度】 Liquid nitrogen vapor phase
【Culture Conditions培養條件】 
【Atmosphere培養環境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培養溫度】 37°C
【STR Profile圖譜】 
【Population Doubling Time倍增殖時間】
【References參考文獻】

更多標記細胞如下：