DSS誘導的腸炎模型
- 公司名稱 上海宇玫博生物科技有限公司
- 品牌
- 型號
- 產地 上海
- 廠商性質 生產廠家
- 更新時間 2017/8/7 10:31:39
- 訪問次數 929
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實驗方案:
1. 將老鼠隨機分組;
2. 對照組每天飲用滅菌水;
3. 模型組每天飲用3.5%DSS至第6天,從第7天開始換為滅菌水,至第12天;
4. 實驗開始后,每天對老鼠進行觀察,稱重和評分;
5. 腸炎觀察指標包括: 體重; 拉稀程度;結腸病變情況等
Brief protocol of DSS induced IBD model
1. Mice 6-8 weeks old, female; Random divided into several groups;
2. DSS concentration 3.5%, control group treated with drink water;
3. DSS treatment DSS in drink water from Day 1 to Day 6 and changed to drink water from Day 7 to Day 12;
4. Observation Mice are observed by measuring body weight twice a week in the first cycle. When obvious weight loss appears, mouse weight is measured on daily basis.
5. Clinical colitis analysis colitis scoring based on Weight loss, Rectal bleeding, Colon shortening, Colon tissue damage.