Roche11745816910 DIG-Nick Translation Mix
參考價 | ¥ 5107.2 |
訂貨量 | ≥1 |
具體成交價以合同協(xié)議為準
- 公司名稱 廣州市齊云生物技術有限公司
- 品牌
- 型號 Roche11745816910
- 產地 美國羅氏
- 廠商性質 代理商
- 更新時間 2017/11/22 0:14:53
- 訪問次數(shù) 1150
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DIG-Nick Translation Mix
Cat.No. | Pack Size | Qty | Price | Options |
---|---|---|---|---|
11745816910 | 160 µl for 40 labeling reactions | 5107.2 |
Application
Used to produce digoxigenin-labeled probes for in situ hybridization.
Product Description
Convenient enzyme and nucleotide mixture. This method utilizes a combination of DNase and DNA Polymerase to nick one strand of the DNA helix, then incorporate labeled nucleotides as the polymerase examines, or “proofreads” the nicked site. Large plasmids, cosmids, and PCR fragments are all regularly used with this method, and the DNA can be linearized or supercoiled. Individual templates produce consistent results in the standard 90-min reaction, and result in an average probe length of 200 base pairs up to 500 base pairs.
Contents
5x conc. solution, stabilized, in reaction buffer with 50% glycerol.
相關分類
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