1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection (WB), 1: 750 (IL)

36-40 | 36-40 for Arabidopsis thaliana

Arabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Kandelia candel, Eriphorum vaginatum, Hordeum vulgare,  Lupinus luteus, Nicotiana tabacum, Picea abies, Pisum sativum,Robinia pseudoacacia, Solanum tuberosum, Solanum esculentum, Physcomitrella patens

Aegilops tauschii, Brachypodium distachyon, Capsella rubella, Citrus sinensis, Citrus clementina, Corylus heterophylla, Crocus sativus, Cucumis sativus, Daucus carota, Glycine max, Hypericum perforatum, Lotus japonicus, Malus x domestica, Medicago truncatula, Medicago sativa, Nicotiana benthamiana, Oryza brachyantha, Oryza sativa, Populus tremula, Picea sitchensis, Triticum aestivum, Saccharum officinarum, S.officinarumea, Sorghum bicolor, Selaginella moellendorffii, Tetrahymena thermophila, Zea mays, Vigna unguiculata, Vitis vinifera

Chlamydomonas reinhardtii

According to Konert et al. (2015) AOX antibody is recognizing AOX1A and AOX1D.

application example 

25 μg of Arabidopsis thaliana mitochondrial wild type fraction (1) mitochondrial fraction from a mutant with increased AOX level (2), total wild type leaf extract (3), total leaf extract from AOX overproducing mutant (4)  were separated on 10% gel and blotted on nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Filters where blocked (1.5h) in 5% milk in TBST (1X TBS, 0,1% Tween 20), incubated with 1: 1000 anti-AOX polyclonal antibodies (2h in TBST) followed by 1 h incubation with 1: 50 000 Agrisera secondary anti-rabbit HRP-coupled antibodies (AS09 602) and visualized with standard ECL on Kodak autoradiography film for 15-60 s. Mitochondria were isolated as described by Urantowka et al. (Plant Mol Biol, 2005, 59:239-52). Mitochondrial pellets were suspended in 1X Laemmli buffer (5% beta-mercaptoetanol, 3.7% glycerol, 1.1% SDS, 23 mM Tris- HCl pH 6.8, 0.01% bromophenol blue), heated (95°C, 5 min.) and centrifuged (13 000rpm, 1 min.). Leaf extracts were prepared as described by Martinez-Garcia et al. (Plant J., 1999, 20:251-7).
Courtesy Dr. Janusz Piechota, Wroc?aw University, Poland