貨號：AS09 413- 植物細胞分裂蛋白FtsZ1/2抗體
| 參考價 | ￥ 3975 |
| 訂貨量 | ≥1 |
- 公司名稱 上海經科化學科技有限公司
- 品牌 Agrisera
- 型號 貨號：AS09 413-
- 產地
- 廠商性質 代理商
- 更新時間 2016/6/2 21:23:05
- 訪問次數 641
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相關抗體：
貨號：AS13 2651---植物細胞分裂蛋白（FtsZ2）抗體
貨號：AS09 413---植物細胞分裂蛋白FtsZ1/2抗體
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兔抗植物細胞分裂蛋白FtsZ1/2抗體介紹：
貨 號：AS09 413
中文名稱：兔抗
英文名稱：FtsZ1 and 2|plant cell division protein抗體 ftsZ1 and ftsZ2
應用：western blot (WB)
規格：50 µl
價格：3975元
兔抗簡介：
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| APPLICATION INFORMATION | ||
| recommended dilution | 1: 2500 with enhanced ECL (WB) | |
| expected | apparent MW | 40 kDa FtsZ1 (Arabidopsis thaliana) and 50 kDa FtsZ2 (Arabidopsis thaliana) | |
| confirmed reactivity | Arabidopsis thaliana, Hordeum vulgare | |
| predicted reactivity | Chlamydomons reinhardtii, Cucumis sativus, Gentiana lutea, Glycine max, Jatropha manihot, Lilium longiflorum, Lupinus angustifolius, Marchantia aquatica, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Ricinus communis, Solanum lycopersicum, Sorgum bicolor, Zea mays, Yellow gentian, Vitis vinifera | |
| not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
| additional information | to be added when available | |
| selected references | to be added when available, antibody released in April 2013. | |
application example
2 µg of total protein extracted with PEB (AS08 300) from leaf tissue of (1) Hordeum vulgare, (2) Arabidopsis thaliana, (3 - 5) recombinant FtsZ1/FtsZ2 (33 kDa) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FtsZ1/FtsZ2 antibody (AS09 413, 1:2500, 1h) and secondary anti-rabbit (1:10 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with TMA-6 (Lumigen) using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.
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