相關(guān)產(chǎn)品：

貨號(hào)：AS09 481---BiP2抗體

貨號(hào)：AS09 481PRE---BiP免疫定位指控 免疫血清

貨號(hào)：AS09 614---BiP抗體 

貨號(hào)：AS09 615---lumenal-binding protein抗體

貨號(hào)：AS09 481---Binding immunoglobulin protein抗體

Binding immunoglobulin protein；BiP2抗體介紹：

貨   號(hào)：AS09 481

中文名稱(chēng)：；BiP2親和純化抗體

英文名稱(chēng)：Binding immunoglobulin protein antibody

應(yīng)用：ELISA (ELISA), western blot (WB), immunofluorescen

規(guī)格：200 µg

價(jià)格：3975元

簡(jiǎn)介：

application example western blot

5 µg of total protein from A.thaliana (1), H. vulgare (2), P. sativum (3)*, Z. mays (4), C. sativus(5), S. tuberosum (6), S. oleracea (7), S. lycopersicum (8) P. patens (9)*, Ch. reinhardtii (10)  extracted with Agrisera PEB extraction buffer (AS08 300) were separated on  4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediay following transfer in  for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from  Agrisera AS09 602) diluted to 1:50 000  for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL  detection reagent according to the manufacturers instructions.  Exposure time was 5 seconds. * Lack of the signal or its low signal intensity in those samples can be due to the sample biology. If you work with those species, please inquire.

application example immunolocalization

BiP localization in 5 days old Arabidopsis thaliana roots (A), 3 days old Triticum aestivum roots (B).
BiP signal shown in red, DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Rabbit anti-BiP primary antibody diluted in 1: 600 and ALEXA 555 conjugated anti-rabbit secondary antibody (red color) have been used. Co-staining with DAPI visualized nucleus (blue color).  Scale bar – 10 µm.